Coding

Part:BBa_K3209000:Design

Designed by: SUN YUHAN   Group: iGEM19_JNFLS   (2019-10-15)


Benzoylformate decarboxylase mutant (BFD-M7)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 771
    Illegal NgoMIV site found at 1233
    Illegal AgeI site found at 235
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 340


Design Notes

The benzoylformate decarboxylase was designed to contain 7 amino acids mutations.


Source

Pseudomonas putida

References